A Rapid, Simple, and High-throughput UPLC-MS/MS Method for Simultaneous Determination of Bioactive Constituents in Salvia Miltiorrhiza with Positive/Negative Ionization Switching

Publication date: Available online 16 August 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Hung-Yu Lin, Ting-Sian Lin, Han-Ju Chien, Yu-Min Juang, Chao-Jung Chen, Chang-Sheng Wang, Chien-Chen LaiAbstractThe dried root of Danshen (Salvia miltiorrhiza) is a Chinese medicine that has been used in traditional treatments for more than 2,000 years in Asia. It has also been used to treat cardiovascular diseases for decades. The goal of this study was to develop a new, fast, and simple ultrahigh-performance liquid chromatography tandem mass spectrometry (UPLC–MS/MS) analytical method for the analysis of phenolic acids and tanshinones in Danshen. The samples were separated on a Kinetex F5 column (2.1 x 100 mm, 1.7 μm, pentafluorophenyl) using 78% acetonitrile with 0.1% formic acid as the mobile phase with isocratic elution while the flow rate was set at 0.45 mL/min. The analysis was performed in a Waters Xevo TQ mass spectrometer via multiple reaction monitoring (MRM) in polarity switching mode. The linearity values of the calibration curves of eight compounds were in the range from 3 to 3200 ng/mL (r2> 0.99). Method validation was performed in terms of linearity, the relative standard deviations (RSDs) of the intra-day and inter-day, and it was found that precisions were within 15% and the accuracy ranged from 93% to 118%. The recovery was more than 87%. The limit of detection (LOD) and limit of quantific...
Source: Journal of Pharmaceutical and Biomedical Analysis - Category: Drugs & Pharmacology Source Type: research

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Publication date: Available online 18 November 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of LipidsAuthor(s): Zhen Wang, Hui Gyu Park, Dong Hao Wang, Riki Kitano, Kumar S.D. Kothapalli, J. Thomas BrennaAbstractBranched chain fatty acids (BCFA) and linear chain/normal odd chain fatty acids (n-OCFA) are major fatty acids in human skin lipids, especially sebaceous gland (SG) wax esters. Skin lipids contain variable amounts of monounsaturated BCFA and n-OCFA, in some reports exceeding over 20% of total fatty acids. Fatty acid desaturase 2 (FADS2) codes for a multifunctional enzyme that catalyz...
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - Category: Lipidology Source Type: research
ConclusionsThe median h-index for an academic thoracic surgeon in Canada and the United States is 10. Surgeons who engage in Twitter activity are more likely to have their research cited by others.
Source: The Annals of Thoracic Surgery - Category: Cardiovascular & Thoracic Surgery Source Type: research
ConclusionsEarly-diastolic phase slope of the HVAD flow waveform might be a novel parameter that can estimate the presence of clinically significant AI.
Source: The Annals of Thoracic Surgery - Category: Cardiovascular & Thoracic Surgery Source Type: research
ConclusionsOnline education for cardiothoracic surgery is seeing increased public use, with high ratings for content and utility. Despite aggressive marketing to STS members, most remain unaware of this website’s existence. Those who are aware approve of its content, but adoption of referring patients to it has been slow. Improved strategies are necessary to make surgeons aware of this STS-provided service and to increase patient referrals to it.
Source: The Annals of Thoracic Surgery - Category: Cardiovascular & Thoracic Surgery Source Type: research
This study evaluated outcomes associated with treatment strategies for this nonagenarian population.MethodsTreatment and overall survival for patients 90 years and older with NSCLC in the National Cancer Data Base (2004-2014) were evaluated using logistic regression, the Kaplan-Meier method, and multivariable Cox proportional hazard models.ResultsThe majority (n = 4152, 57.6%) of the 7205 patients 90 years or older with stage I-IV NSCLC did not receive any therapy. For the entire cohort, receiving treatment was associated with significantly better survival when compared with no therapy (5-year survival, 9.3% [95% confidenc...
Source: The Annals of Thoracic Surgery - Category: Cardiovascular & Thoracic Surgery Source Type: research
ConclusionsThe delay in surgical resection of StageIIIa NSCLC is not associated increased early mortality, however it is associated with worse 3-year post-resection survival.
Source: The Annals of Thoracic Surgery - Category: Cardiovascular & Thoracic Surgery Source Type: research
Publication date: March 2020Source: Interdisciplinary Neurosurgery, Volume 19Author(s): Joel Kaye, Ahmed Meleis, Shabbar Danish, Zhenggang XiongAbstractMeningiomas are relatively slow-growing, typically benign tumors that arise from the arachnoid cells which form the meninges. They are the most common primary brain tumor and account for nearly one-third of all primary brain and spine tumors. Meningiomas can arise from any dural site, however they are infrequently found at the cerebellopontine angle (CPA). While WHO grade I meningiomas are benign and the slowest growing, WHO grade II meningiomas grow significantly faster, d...
Source: Interdisciplinary Neurosurgery - Category: Neurosurgery Source Type: research
We present a case of Moyamoya disease in an infant who presented with neurological symptoms including convulsions and motor deficits. He was misdiagnosed two times and finally investigated in a tertiary hospital with brain computed tomography and magnetic resonance imaging showing affected vessels with typical pattern of Moyamoya diseases in the brain. He underwent surgical intervention and scheduled for follow up.
Source: Interdisciplinary Neurosurgery - Category: Neurosurgery Source Type: research
ConclusionsIn the current series, the factors which were associated with an increased rate of iatrogenic pituitary injury were: increased manipulation of the gland intra-operatively, a firm tumor consistency not amenable to suction or curettage, and larger pituitary adenoma size. Recognition of the heterogeneity of macroadenoma consistency and growth patterns and appropriate usage of intra- and/or extra-capsular tumor resection techniques may help mitigate the risk of surgical pituitary injury.
Source: Interdisciplinary Neurosurgery - Category: Neurosurgery Source Type: research
Conclusion. The hexane extract of L. apiculata leaves showed the lower MIC against S. aureus when compared with extracts obtained from other parts of the plant. The growth curve and the colonies count suggest a bacteriostatic activity of the L. apiculata leaves extract against Staphylococcus aureus. PMID: 31737073 [PubMed]
Source: International Journal of Microbiology - Category: Microbiology Tags: Int J Microbiol Source Type: research
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