Synthesis of an oleic acid based pH-responsive lipid and its application in nanodelivery of vancomycin

Publication date: Available online 17 August 2018Source: International Journal of PharmaceuticsAuthor(s): Danford Mhule, Rahul S. Kalhapure, Mahantesh Jadhav, Calvin A. Omolo, Sanjeev Rambharose, Chunderika Mocktar, Sanil Singh, Ayman Y. Waddad, Valence M.K Ndesendo, Thirumala GovenderAbstractStimuli-responsive nano-drug delivery systems can optimize antibiotic delivery to infection sites. Identifying novel lipids for pH responsive delivery to acidic conditions of infection sites will enhance the performance of nano-drug delivery systems. The aim of the present investigation was to synthesize and characterize a biosafe novel pH-responsive lipid for vancomycin delivery to acidic conditions of infection sites. A pH-responsive solid lipid, N-(2-morpholinoethyl) oleamide (NMEO) was synthesized and used to prepare vancomycin (VCM)-loaded solid lipid nanoparticles (VCM_NMEO SLNs). The particle size (PS), polydispersity index (PDI), zeta potential (ZP) and entrapment efficiency (EE) of the formulation were 302.8 ± 0.12 nm, 0.23 ± 0.03, -6.27 ± 0.017 mV and 81.18 ± 0.57 % respectively. The study revealed that drug release and antibacterial activity were significantly greater at pH 6.0 than at pH 7.4, while the in silico studies exposed the molecular mechanisms for improved stability and drug release. Moreover, the reduction of MRSA load was 4.14 times greater (p <0.05) in the skin of VCM_NMEO SLNs treated mice than that of bare VCM treated specimens. Thus, this study confirme...
Source: International Journal of Pharmaceutics - Category: Drugs & Pharmacology Source Type: research