Soluble dietary fiber from Qing Ke (highland barley) brewers spent grain could alter the intestinal cholesterol efflux in Caco-2 cells

Publication date: August 2018Source: Journal of Functional Foods, Volume 47Author(s): Huijuan Zhang, XinRan Cao, Meng Yin, Jing WangAbstractThe present study investigated the antioxidant activities and hypocholesterolemic properties of soluble dietary fiber (SDF) obtained from Qing Ke brewer’s spent grain using Caco-2 cells. SDF simultaneously increased the activities and mRNA expression levels of the phase II detoxifying/antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidise (GPx). The expression levels of the intestinal genes for cholesterol efflux, ABCA1, ABCG5, and LXRα, were up-regulated by treatment with SDF. Moreover, SDF decreased the transcriptional levels of genes related to cholesterol absorption and synthesis in Caco-2 cells. The results suggested that hypocholesterolemic effects of SDF may be due, at least in part, to its antioxidant activities.Graphical abstract
Source: Journal of Functional Foods - Category: Nutrition Source Type: research