Establishment and characterization of a cell line from a feline histiocytic sarcoma

Publication date: July 2018Source: Veterinary Immunology and Immunopathology, Volume 201Author(s): Ryo Miyamoto, Sena Kurita, Hiroyuki Tani, Masato Kobayashi, Soudai Sugiura, Kae Shigihara, Yuka Sato, Yoshikazu Tanaka, Kyoichi Tamura, Makoto BonkobaraAbstractFeline histiocytic sarcoma (HS) is an aggressive and uncommon tumor originating from dendritic cells/macrophages. Here, a feline HS cell line, FHS-1, was established from a case of feline HS and characterized. Immunohistochemically, FHS-1 cells were positive for vimentin and Iba-1, and negative for MHC class II and CD163. FHS-1 cells were positive for α-naphthyl butyrate esterase staining, which was clearly inhibited by sodium fluoride. FHS-1 cells had phagocytic and antigen uptake/processing activities. Moreover, FHS-1 cells were tested for susceptibility to feline infectious peritonitis virus (FIPV) strain 79-1146; however, this cell line was not susceptible to this viral strain. Although FHS-1 cells lost the expression of MHC class II and CD163, our findings indicate that FHS-1 is a feline HS cell line that retains functional properties of dendritic cells/macrophages in terms of phagocytic and antigen uptake/processing activities. While FHS-1 cells are not suitable for in vitro study of FIP using strain 79-1146, they may be applicable for studies aimed at developing new diagnostic and therapeutic strategies for feline HS.
Source: Veterinary Immunology and Immunopathology - Category: Veterinary Research Source Type: research