The protective role of Trpc6 knockout in the progression of diabetic kidney disease.

The protective role of Trpc6 knockout in the progression of diabetic kidney disease. Am J Physiol Renal Physiol. 2018 Jun 20;: Authors: Spires D, Ilatovskaya DV, Levchenko V, North PE, Geurts AM, Palygin O, Staruschenko A Abstract Diabetic kidney disease (DKD) is a chronic kidney pathology that leads to end stage renal disease. Previous studies from our laboratory indicate that there is an association between the development of DKD and the TRPC6 channel. Trpc6 expression and activity was increased in the streptozotocin (STZ)-treated Dahl Salt-sensitive (SS) rat, an established model of type 1 diabetes. Here, using a Trpc6 knock-out created on the SS rat background (SSTRPC6-/-), we test the hypothesis that the absence of Trpc6 will protect podocytes and kidney function during the development of DKD. Four groups of animals - control SSWT, SSTRPC6-/-, STZ-treated SSWT and STZ-SSTRPC6-/- - were utilized in this study. Diabetes development was monitored for 11 weeks after STZ injection with periodic weight, glucose, and urinary output measurements. There was an increase in albuminuria and glomerular injury following STZ treatment, which was not different between SS and SSTRPC6-/- groups. Western blot analysis revealed elevated levels of nephrin in urine samples of STZ-SSWT rats, which was higher compared to STZ-SSTRPC6-/- rats. Furthermore, pathological increases in basal [Ca2+]i levels and foot process damage of podocytes during the deve...
Source: Am J Physiol Renal P... - Category: Urology & Nephrology Authors: Tags: Am J Physiol Renal Physiol Source Type: research