Genistein Promotes Proliferation of Human Cervical Cancer Cells Through Estrogen Receptor-Mediated PI3K/Akt-NF- κ B Pathway
Phytoestrogens are polyphenol compounds which have similar structure to 17β-estradiol (E2), a kind of main estrogen in women. Thus, phytoestrogens may affect the reproductive and endocrine systems, leading to the development of estrogen-related cancers. The effect of genistein (Gen), one of the most studied phytoestrogens, on human cervical cancer cells (HeLa) was investigated in this study. It was found that Gen at concentrations of 0.001, 0.01, 0.1 and 1 µmol·L-1 promoted the proliferation of HeLa cells in a dose-dependent manner. Gen increased the portion of HeLa cells in S phase and decreased the portion of the cells in G1 phase. Besides, apoptosis rate of the cells was significantly lower when treated with Gen compared with the control group. It was also found that the expression of ERα, Akt or nuclear NF-κB p65 protein was activated by Gen. The correlation between these three proteins may be as following: ERα was the upstream, followed by Akt, and then nuclear NF-κB p65 protein. In addition, the downstream genes of activated nuclear NF-κB p65 were found to be associated with cell cycle and apoptosis of cancer cells. Our results suggested that Gen may stimulate cell proliferation partially through the estrogen receptor-mediated PI3K/Akt-NF-κB pathway and the further activation of the downstream genes of nuclear NF-κB p65.
Publication date: September–October 2019Source: Journal of the American Society of Cytopathology, Volume 8, Issue 5Author(s): Caddie Laberiano, Wilmer Silva Caso
Conditions: Uterine Cervical Neoplasms; Cervical Cancer; Cervical Neoplasms; Cervix Cancer Interventions: Drug: Sintilimab; Drug: quadrivalent HPV vaccine Sponsors: Buhai Wang; Innovent Biologics (Suzhou) Co. Ltd. Recruiting
Condition: Cervical Cancer, Endometrial Cancer，Endometrial Lesion&Adnexal Lesion ，Ectopic Pregnancy&Infertility ，Pelvic Obstructive Disorder&Genital Tract Deformity Intervention: Sponsors: Peking Union Medical College Hospital; Chinese Medical Doctor Association; Chinese Obstetricians and Gynecologists Association Recruiting
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ConclusionsTranscervical pharyngo-laryngo-cervico-esophagectomy should be considered in patients with isolated cervical esophageal tumors. Pharyngo-laryngo-cervico-esophagectomy allows adequate tumor resection while preserving the esophagus and stomach below. Operative morbidity and mortality outcomes were improved without compromising oncologic control.
Conclusions: A well-designed health educational interventional program using peer educators could be a successful approach to create awareness on sensitive topics.
As women's healthcare clinics continue to close in the United States, cervical cancer screening rates have fallen, showing a trend toward diagnosis for late stage disease and a higher risk of death.Medscape Medical News