Direct Attachment of Nanoparticle Cargo to Salmonella typhimurium Membranes Designed for Combination Bacteriotherapy Against Tumors

Nanoparticle technology is an emerging approach to resolve difficult-to-manage internal diseases. It is highly regarded, in particular, for medical use in treatment of cancer due to the innate ability of certain nanoparticles to accumulate in the porous environment of tumors and to be toxic to cancer cells. However, the therapeutic success of nanoparticles is limited by the technical difficulty of fully penetrating and thus attacking the tumor. Additionally, while nanoparticles possess seeming-specificity due to the unique physiological properties of tumors themselves, it is difficult to tailor the delivery of nanoparticles or drugs in other models, such as use in cardiac disease, to the specific target. Thus, a need for delivery systems that will accurately and precisely bring nanoparticles carrying drug payloads to their intended sites currently exists. Our solution to this engineering challenge is to load such nanoparticles onto a biological “mailman” (a novel, nontoxic, therapeutic strain of Salmonella typhimurium engineered to preferentially and precisely seek out, penetrate, and hinder prostate cancer cells as the biological delivery system) that will deliver the therapeutics to a target site. In this chapter, we describe two methods that establish proof-of-concept for our cargo loading and delivery system by attaching nanoparticles to the Salmonella membrane. The first method (Subheading 1.1) describes association of sucrose-conjugated gold nanoparticles to...
Source: Springer protocols feed by Infectious Diseases - Category: Infectious Diseases Source Type: news

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Authors: Eisenstark A Abstract Prostate cancer remains a life-threatening disease of men. While early detection has been helpful to reduce the mortality rate, we currently do not have a desired therapy. In recent years, new strategies have been proposed to treat prostate cancers with poor prognosis by utilizing genetically modified bacteria, including Salmonella typhimurium that preferentially replicate within solid tumors (1000:1 and up to 10,000:1 compared to non-cancerous tissue) destroying cancer cells without causing septic shock that is typically associated with wild-type S. typhimurium infections. Furthermor...
Source: Advances in Experimental Medicine and Biology - Category: Research Tags: Adv Exp Med Biol Source Type: research
In Vivo Study of the Effects of ERβ on Apoptosis and Proliferation of Hormone-Independent Prostate Cancer Cell Lines PC-3M. Biomed Res Int. 2018;2018:1439712 Authors: Zhou C, Yu C, Guo L, Wang X, Li H, Cao Q, Li F Abstract Objective: To evaluate the in vivo therapeutic effects of attenuated Salmonella carrying PCDNA3.1-ERβ plasmid in hormone-independent prostatic cancer in nude mice and to clarify the mechanism by which estrogen receptor β (ERβ) induces apoptosis and proliferation in prostatic cancer cells in mice. Methods: The orthotopic prostatic cancer models of mice were r...
Source: Biomed Res - Category: Research Authors: Tags: Biomed Res Int Source Type: research
In what is believed to be one of the first deaths from an e-cigarette explosion, a 38-year-old man in Florida was killed when his vape pen exploded, sending projectiles into his head and causing a small fire in his house. Tallmadge D’Elia was found May 5 in the burning bedroom of his family’s home in […]Related:More men with low-risk prostate cancer are forgoing aggressive treatmentEgg farm responsible for salmonella outbreak had ‘unacceptable rodent activity,’ FDA report saysEbola has infected dozens so far in Congo, killing 19, WHO says
Source: Washington Post: To Your Health - Category: Consumer Health News Source Type: news
A 38-year-old man in Florida was killed when his vape pen exploded, sending projectiles into his head and causing a small fire in his house, in what is believed to be one of the first deaths from an e-cigarette explosion. Tallmadge D’Elia was found May 5 in the burning bedroom of his family’s home in […]Related:More men with low-risk prostate cancer are forgoing aggressive treatmentEgg farm responsible for salmonella outbreak had ‘unacceptable rodent activity,’ FDA report saysEbola has infected dozens so far in Congo, killing 19, WHO says
Source: Washington Post: To Your Health - Category: Consumer Health News Source Type: news
A North Carolina egg farm that authorities say is responsible for an outbreak of salmonella illness that has sickened several people in nine states has had a heavy rodent infestation and failed to take actions to reverse it, according to an inspection report. Dozens of rodents, some alive and some dead, were found inside Rose Acre Farms’ […]Related:More men with low-risk prostate cancer are rejecting aggressive treatment, new study suggestsEbola has infected dozens so far in Congo, killing 19, WHO saysTroubling link found between pollution exposure in pregnancy, high blood pressure in children
Source: Washington Post: To Your Health - Category: Consumer Health News Source Type: news
Contributors : Vadim Mett ; Andrei GudkovSeries Type : Expression profiling by arrayOrganism : Mus musculusToll-like receptor 5 is considered an attractive target for anticancer immunotherapy. TLR5 agonists, bacterial flagellin and engineered flagellin derivatives, have been shown to have potent anti-tumor and metastasis-suppressive effects in multiple animal models and to be safe in both animals and humans. Anticancer efficacy of TLR5 agonists stems from TLR5-dependent activation of NF-kB that mediates innate and adaptive anti-tumor immune responses. To extend application of TLR5-targeted anticancer immunotherapy to tumor...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by array Mus musculus Source Type: research
Abstract Chlordecone (CLD) is a chlorinated hydrocarbon insecticide, now classified as a persistent organic pollutant. Several studies have previously reported that chronic exposure to CLD leads to hepatotoxicity, neurotoxicity, raises early child development and pregnancy complications, and increases the risk of liver and prostate cancer. In situ chemical reduction (ISCR) has been identified as a possible way for the remediation of soils contaminated by CLD. In the present study, the objectives were (i) to evaluate the genotoxicity and the mutagenicity of two CLD metabolites formed by ISCR, CLD-5a-hydro, or CLD-5...
Source: Environmental Science and Pollution Research International - Category: Environmental Health Authors: Tags: Environ Sci Pollut Res Int Source Type: research
Authors: Altıntaş Kazar G, Şen E Abstract Basic applications in cancer therapy may fail to eradicate cancer cells completely, they can show toxic affects to healthy cells and development of resistance to antitumor agents may increase tendency to metastasis. Bacterial therapies have the advantage of specific targetting of tumors by selective toxicity, responsiveness to external signals, self-propelling capacity, and the sense of microenvironment. The most interest on the bacterial cancer therapy is about Salmonella spp. with a special emphasis of S.Typhimurium. The aim of this study was to investigate the antitum...
Source: Mikrobiyoloji Bulteni - Category: Microbiology Tags: Mikrobiyol Bul Source Type: research
Authors: Toneri M, Miwa S, Zhang Y, Hu C, Yano S, Matsumoto Y, Bouvet M, Nakanishi H, Hoffman RM, Zhao M Abstract Bone metastasis is a frequent occurrence in prostate cancer patients and often is lethal. Zoledronic acid (ZOL) is often used for bone metastasis with limited efficacy. More effective models and treatment methods are required to improve the outcome of prostate cancer patients. In the present study, the effects of tumor-targeting Salmonella typhimurium A1-R were analyzed in vitro and in vivo on prostate cancer cells and experimental bone metastasis. Both ZOL and S. typhimurium A1-R inhibited the growth o...
Source: Oncotarget - Category: Cancer & Oncology Tags: Oncotarget Source Type: research
The transcriptional profile of PC-3 human prostate cancer cells infected with tumor-targeting Salmonella typhimurium A1-R (A1-R) was analyzed with by microarray. PC-3 is highly sensitive to A1-R. Numerous genes involved in diverse cellular processes, including apoptosis, necrotic cell death, neutrophil chemotaxis and innate immunity were significantly up-regulated by A1-R bacterial infection. Such up-regulated genes include TNF-a, Fos and JUN which are involved in cell death and IL-1A and CXCL which are involved in neutrophil chemotaxes. Knowledge of the up-regulated gene profile will be exploited for enhancing prostate tu...
Source: Cancer Research - Category: Cancer & Oncology Authors: Tags: Molecular and Cellular Biology Source Type: research
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