Development and storage studies of high density macrocapsules containing Lactobacillus spp. strains as nutritional supplement in young calves.

Development and storage studies of high density macrocapsules containing Lactobacillus spp. strains as nutritional supplement in young calves. Rev Argent Microbiol. 2018 Mar 17;: Authors: Astesana DM, Zimmermann JA, Frizzo LS, Zbrun MV, Blajman JE, Berisvil AP, Romero-Scharpen A, Signorini ML, Rosmini MR, Soto LP Abstract The aim of this study was to evaluate different production methodologies of probiotic macrocapsules with high bacterial densities destined to lactating calves. Three types of capsules containing Lactobacillus casei DSPV318T and Lactobacillus plantarum DSPV354T were prepared from an overnight culture in whey medium: (1) mixing the culture with calcium alginate and then, reincubating the capsules in whey (RC); (2) concentrating the biomass by centrifugation and mixing the pellet with calcium alginate (CC) at different concentrations with respect to the initial culture (5X and 12.5X); (3) CC with cryoprotectants: whey permeate (Per) and glycerol (Gly). Chitosan coating was evaluated. Capsules were freeze-dried and viability was assessed before freezing, after freeze-drying and every two weeks for 84 days of storage at room temperature, 4°C and -20°C. CC showed higher cell densities than RC. Storage temperature affected viability: greater viability at lower temperature. Moreover, the effect of temperature was influenced by other factors, such as capsule coating, culture neutralization and cryoprotectants. Coating impr...
Source: Revista Argentina de Microbiologia - Category: Microbiology Tags: Rev Argent Microbiol Source Type: research