Preparation and characterization of gelatin-chitosan-nano β-TCP based scaffold for orthopaedic application.

Preparation and characterization of gelatin-chitosan-nanoβ-TCP based scaffold for orthopaedic application. Mater Sci Eng C Mater Biol Appl. 2018 May 01;86:83-94 Authors: Maji K, Dasgupta S, Pramanik K, Bissoyi A Abstract The primary aim of this study was to fabricate gelatin/chitosan/β-TCP (GCT) composite scaffold to improve its compressive mechanical behaviour and in-vivo biocompatibility with predictable degradation rate. Beta tricalcium phosphate (β-TCP) powder was synthesized in size range between 70-100 nm using aqueous precipitation route at a fixed Ca/P molar ratio of 1.5:1 at pH 10 and after subsequent heat treatment of as precipitated powder at 800 °C for 4 hours. The composite scaffolds were fabricated using solid-liquid phase separation of the slurry containing gelatin, chitosan, β-tricalcium phosphate in varying proportion and subsequent lyophilisation of the phase separated mixture. The prepared scaffolds exhibited high porosity (>80%) with pore sizes ranging between 78-382 μm as determined using Hg-porosimetry. SEM result revealed that incorporation of β-TCP to the extent of 30 wt% resulted in well-shaped and uniformly distributed interconnected pores of average pore size of 120 ± 18.6 μm in it. Compressive strength of the scaffolds was increased from 0.8 MPa to 2.45 MPa on increase in β-TCP content from 10 wt%-30 wt% in the prepared scaffold. Human Umbilical Cord derived mesenchymal...
Source: Appl Human Sci - Category: Physiology Authors: Tags: Mater Sci Eng C Mater Biol Appl Source Type: research