The regulation and function of microRNA-377/RASSF8 signaling axis in gastric cancer.

The regulation and function of microRNA-377/RASSF8 signaling axis in gastric cancer. Oncol Lett. 2018 Mar;15(3):3630-3638 Authors: Bo X, Chen Y, Sheng W, Gong Y, Wang H, Gao W, Zhang B Abstract Gastric cancer is a major cause of cancer-associated mortality worldwide. The aberrant expression of microRNA (miRNA) is involved in tumorigenesis. Ras proteins transfer information from the extracellular environment to internal cellular compartments and are essential in numerous signal transduction pathways. To investigate the regulation, function and clinical significance of the miRNA377/Ras association domain family (RASSF) 8 signaling axis in gastric cancer, reverse transcription-quantitative polymerase chain reaction, immunohistochemistry, cell counting kit-8, western blotting, and Transwell assays were used. The results revealed that expression of RASSF8 was significantly upregulated in normal gastric tissues compared with gastric cancer, which was further confirmed by immunohistochemical analysis, and its expression level was increased in normal gastric cells compared with gastric cancer cell lines. However, the expression of miR-377 was significantly upregulated in gastric cancer compared with normal gastric tissues. In addition, RASSF8 overexpression in BGC-823 gastric cancer cells significantly inhibited the proliferation, apoptosis and invasive abilities of cells. Whereas miR-377 attenuated these effects due to downregulated RASSF8 ...
Source: Oncology Letters - Category: Cancer & Oncology Tags: Oncol Lett Source Type: research