Characterization of Corneal Endothelial Injury During Penetrating Keratoplasty

Purpose: To characterize corneal endothelial injury during penetrating keratoplasty in a controlled wet laboratory environment using human tissue. To identify potential areas or steps within surgery in which insult to the corneal endothelium may be most affected by trauma during routine penetrating keratoplasty. Methods: Human donor corneas (n = 12) with intact endothelium underwent experimental penetrating keratoplasty. Endothelial injury was evaluated after each suture quartile using trypan staining, a validated modality for assessing endothelial injury. Insult was quantified using high-resolution photography and computer software. Results: Statistical significance was found in the change in staining between quartiles as determined by repeated-measures analysis of variance (F3,11 = 5.83, P
Source: Cornea - Category: Opthalmology Tags: Clinical Science Source Type: research