NGAL gene silencing inhibits proliferation and promotes apoptosis of human gastric cancer cells: an in vivo and in vitro study

This study aims to explore effects of neutrophil gelatinase‐associated lipocalin (NGAL) gene silencing on the proliferation and apoptosis of human gastric cancer MGC‐803 and SGC‐7901 cells. NGAL‐siRNA was designed, synthesized and randomly divided into NGAL‐siRNA and control‐siRNA groups. Gastric cancer cells MGC‐803 and SGC‐7901 were grouped into blank, NGAL‐OE, and control‐OE groups. Expression of NGAL and apoptosis‐related proteins were detected by qRT‐PCR and Western blotting. Cell cycle and apoptosis were tested by flow cytometry, and cell proliferation by water soluble tetrazolium‐1 (WST‐1) assay. The effect of NGAL gene silencing on tumorigenicity of MGC‐803 and SGC‐7901 cells in vivo was detected through establishment of xenograft model in nude mice. Compared with the blank and control‐siRNA groups, expression of NGAL gene were obviously reduced, and the amount of MGC‐803 and SGC‐7901 cells in G0/G1 phase increased while the number of cells in S phase reduced in the NGAL‐siRNA group; the proliferation was inhibited, while its apoptosis was enhanced; the expression of p65, p‐p65 and Bcl‐2 were lower and the expressions of caspase‐9 and Bax were higher; while the NGAL‐OE group had opposite trend. The tumor‐bearing nude mice experiment showed that NGAL gene silencing inhibited the proliferation and tumorigenicity of the MGC‐803 and SGC‐7901 in vivo, which was further supported by lower proliferative cell nuclear antig...
Source: Journal of Cellular Biochemistry - Category: Biochemistry Authors: Tags: Article Source Type: research