Correlative imaging reveals physiochemical heterogeneity of microcalcifications in human breast carcinomas

Publication date: Available online 6 December 2017 Source:Journal of Structural Biology Author(s): Jennie A.M.R. Kunitake, Siyoung Choi, Kayla X. Nguyen, Meredith M. Lee, Frank He, Daniel Sudilovsky, Patrick G. Morris, Maxine S. Jochelson, Clifford A. Hudis, David A. Muller, Peter Fratzl, Claudia Fischbach, Admir Masic, Lara A. Estroff Microcalcifications (MCs) are routinely used to detect breast cancer in mammography. Little is known, however, about their materials properties and associated organic matrix, or their correlation to breast cancer prognosis. We combine histopathology, Raman microscopy, and electron microscopy to image MCs within snap-frozen human breast tissue and generate micron-scale resolution correlative maps of crystalline phase, trace metals, particle morphology, and organic matrix chemical signatures within high grade ductal carcinoma in situ (DCIS) and invasive cancer. We reveal the heterogeneity of mineral-matrix pairings, including punctate apatitic particles (<2 µm) with associated trace elements (e.g., F, Na, and unexpectedly Al) distributed within the necrotic cores of DCIS, and both apatite and spheroidal whitlockite particles in invasive cancer within a matrix containing spectroscopic signatures of collagen, non-collagen proteins, cholesterol, carotenoids, and DNA. Among the three DCIS samples, we identify key similarities in MC morphology and distribution, supporting a dystrophic mineralization pathway. This multimodal methodology lays ...
Source: Journal of Structural Biology - Category: Biology Source Type: research

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We examined this approach for studies on several types of T cell membrane proteins, previously defined as raft or non-raft associated, to see whether the properties of the raft derived SMALPs differ from non-raft SMALPs. Our results indicate that two types of raft proteins, GPI-anchored proteins and two Src family kinases, are markedly present in membrane fragments much larger (>250 nm) than those containing non-raft proteins (
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - Category: Biochemistry Source Type: research
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Source: AllAfrica News: Health and Medicine - Category: African Health Source Type: news
Abstract Construction of hybrid systems that combine the cancer treatment and diagnosis agents on a single platform, known as theranostic systems, have received great attentions in the field of nanobiomedicine. Here, construction and characterization of a new multifunctional hybrid theranostic system based on RGO, PDA, BSA, DTPA-Mn(II), and MTX constituents, is presented. Accordingly, GO is partially reduced and simultaneously functionalized by dopamine, leading to reduced graphene oxide/polydopamine, RGO-PDA system; and then, the bovine serum albumin protein (BSA) is grafted onto this system. The obtained system,...
Source: Biosensors and Bioelectronics - Category: Biotechnology Authors: Tags: Biosens Bioelectron Source Type: research
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Source: Daily Express - Health - Category: Consumer Health News Source Type: news
Conclusions: The heterogeneous effect of state legislation with regard to dense breast tissue on screening mammography follow-up by ultrasound may be explained by specific and unique characteristics of the approaches taken by a variety of states.
Source: Medical Care - Category: Health Management Tags: Original Articles Source Type: research
Publication date: Available online 13 August 2018Source: European Journal of Surgical OncologyAuthor(s): João Gonçalves Filho, Mark E. Zafereo, Faisal I. Ahmad, Iain J. Nixon, Ashok R. Shaha, Vincent Vander Poorten, Alvaro Sanabria, Avi Khafif Hefetz, K. Thomas Robbins, Dipti Kamani, Gregory W. Randolph, Andres Coca-Pelaz, Ricard Simo, Alessandra Rinaldo, Peter Angelos, Alfio Ferlito, Luiz P. KowalskiAbstractThe central compartment is a common site for nodal spread from differentiated thyroid carcinoma, often occurring in patients without clinical or ultrasonographic (US) evidence of neck lymph node metastasi...
Source: European Journal of Surgical Oncology (EJSO) - Category: Surgery Source Type: research
Conclusion: Margin positivity after resection for noninvasive IPMNs is primarily due to low grade dysplasia and is not associated with developing recurrence in the remnant pancreas or at the resection margin. Long-term surveillance is required for all patients, as a significant number of recurrences developed over 5 years after the index operation.
Source: Annals of Surgery - Category: Surgery Tags: Papers of the 138Th ASA Annual Meeting Source Type: research
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Source: IPS Inter Press Service - Health - Category: International Medicine & Public Health Authors: Tags: Africa Development & Aid Environment Food & Agriculture Headlines Health Humanitarian Emergencies Population Poverty & SDGs Source Type: news
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Source: Cancer Research - Category: Cancer & Oncology Authors: Tags: Invited Speaker Abstracts Source Type: research
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Source: Cancer Research - Category: Cancer & Oncology Authors: Tags: Poster Session Abstracts Source Type: research
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