Anonna muricata L. (soursop) seed oil improves type 1 diabetes parameters in vivo and in vitro

In this study, we identified the immunomodulatory potential of A. muricata seed oil (AmSO) in streptozotocin (STZ)-induced in vivo and in vitro experimental model of T1D and whole blood cell culture from diabetic patients. AmSO cytotoxicity was evaluated by MTT-tetrazolium and resazurin. AmSO was orally administered to BALB/c mice for 48days and were divided into groups: control, STZ (diabetic), STZ–AmSO (diabetic, treated with 1.0mg/kg AmSO), and AmSO groups (nondiabetic, treated with 1.0mg/kg AmSO). T1D was induced with intraperitoneal administration of STZ (3×100mg/Kg). Biochemistry and histopathological analysis and area of pancreatic islets were evaluated. IL-10, IL-4, IL-17 production in spleen cell culture from diabetic mice exposed to AmSO and IFN-γ and IL-10 levels in whole blood cell culture from diabetic patients exposed to AmSO was determined by ELISA. AmSO showed antihyperglycemic effect, preserved the area of ​​pancreatic islets, preserved liver tissue, increased IL-4 and IL-10 levels in spleen cell culture, and decreased IFN-γ level in whole blood cell culture. AmSO demonstrated immunomodulatory effect and therapeutic potential for the treatment and/or prevention of clinical T1D. Graphical abstract
Source: PharmaNutrition - Category: Nutrition Source Type: research