A simple, cost-effective method for generating murine colonic 3D enteroids and 2D monolayers for studies of primary epithelial cell function

We describe, in detail, the protocols required to maintain three-dimensional cultures of murine colonoids and passage these primary epithelial cells to confluent monolayers in a standardized, high-yield and cost-effective manner.

Source: AJP: Gastrointestinal and Liver Physiology - November 1, 2017 Category: Gastroenterology Authors: Fernando, E. H., Dicay, M., Stahl, M., Gordon, M. H., Vegso, A., Baggio, C., Alston, L., Lopes, F., Baker, K., Hirota, S., McKay, D. M., Vallance, B., MacNaughton, W. K. Tags: INNOVATIVE METHODOLOGY Source Type: research