Abstract P265: Placental Ischemia Causes Renal Mitochondrial Impairment in Reduced Uterine Perfusion Pressure Rats [Session Title: Onsite Poster Competition II]

Introduction: Preeclampsia (PE) is characterized by new onset hypertension in pregnancy and is associated with renal dysfunction, proteinuria and is believed to be initiated by placental ischemia. Mitochondrial dysfunction is an important source of reactive oxygen species (ROS) generation. To better understand the role of mitochondrial dysfunction in hypertension during PE we examined renal mitochondrial expression in the kidney of RUPP rats.Methods: Female Sprague Dawley rats were dived into two groups; normal pregnant (NP) and RUPP rats. On gestational day (GD) 14, RUPP surgery was performed, GD18 carotid catheters were inserted, and GD19 conscious blood pressure (MAP) was measured, renal mitochondria were isolated for respiration and analysis. Respiration measurements were performed on intact isolated mitochondria under glutamate/malate as complex I substrate using Oroboros Oxygraph-2K. Oxidative phosphorylation was analyzed by western blot using total Oxphos cocktail antibody and VDAC.Results: MAP was elevated in RUPP (n=8) vs NP rats (n=10) (125±6 vs. 99±2 mmHg, p
Source: Hypertension - Category: Cardiology Authors: Tags: Poster Abstract Presentations Source Type: research