Vasodilator effect of Angiotensin- (1-7) on vascular coronary bed of rats: Role of Mas, ACE and ACE2.

Vasodilator effect of Angiotensin- (1-7) on vascular coronary bed of rats: Role of Mas, ACE and ACE2. Protein Pept Lett. 2017 Jul 28;: Authors: Moraes PL, Kangussu LM, Castro CH, Santos RA, Ferreira AJ, Almeida AP Abstract Angiotensin(Ang)-(1-7) is a biologically active member of the renin-angiotensin system that participates of the regulation of blood pressure. Although Ang-(1-7) is able to potentiate the vasodilator effect of bradykinin in coronary bed of rats, a direct vasodilator effect of Ang-(1-7) in this vascular bed has not been characterized. Thus, the aim of this study was to evaluate the mechanisms involved in the vasodilator effect of Ang-(1-7) in the vasculature of isolated rat hearts perfused according to the Langendorff technique at constant flow. After approximately 30 minutes of stabilization, hearts were perfused with Krebs-Ringer solution (KRS) alone (control) or KRS containing Ang-(1-7). The participation of the Ang-(1-7) receptor Mas, AT1 receptor, angiotensin-converting enzyme (ACE) and ACE2 was evaluated perfusing hearts with a combination of Ang-(1-7) plus A-779, Ang-(1-7) plus losartan, Ang-(1-7) plus captopril/enalapril and Ang-(1-7) plus DX-600, respectively. Ang-(1-7) induced a significant decrease in the perfusion pressure, indicating a direct vasodilatation action of this peptide in the coronary bed. This effect was abolished by A779, captopril, enalapril and DX-600, ACE2-specific inhibitor. However, AT1...
Source: Protein and Peptide Letters - Category: Biochemistry Authors: Tags: Protein Pept Lett Source Type: research