Comparative evaluation of fluorescent in situ hybridization and Giemsa microscopy with quantitative real-time PCR technique in detecting malaria parasites in a holoendemic region of Kenya

Early and accurate diagnosis of malaria is important in treatment as well as in the clinical evaluation of drugs and vaccines. Evaluation of Giemsa-stained smears remains the gold standard for malaria diagnosi...
Source: Malaria Journal - Category: Infectious Diseases Authors: Source Type: research

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J. L. Abbate et al.
Source: Emerging Infectious Diseases Journal - Category: Infectious Diseases Source Type: research
Development of effective malaria vaccines requires delivery platforms to enhance the immunogenicity and efficacy of the target antigens. This is particularly challenging for transmission-blocking malaria vaccines (TBVs), and specifically for those based on the Pfs25 antigen, that need to elicit very high antibody titers to stop the parasite development in the mosquito host and its transmission. Presenting antigens to the immune system on virus-like particles (VLPs) is an efficient way to improve the quantity and quality of the immune response generated. Here we introduce for the first time a new VLP vaccine platform, based...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
Publication date: Available online 11 December 2019Source: Journal of Molecular StructureAuthor(s): Terence J. Noonan, Kelly Chibale, Susan A. Bourne, Mino R. CairaAbstractA crystal engineering approach combined with experimental screening methods was employed as a preformulation step for producing expectedly useful forms of a novel antimalarial drug lead (MMV652103). In previous experiments, this drug lead had shown promise both in vitro and in vivo against the human malaria parasite Plasmodium falciparum. Various new forms of MMV652103 were produced earlier, including co-crystals, a salt, polymorphs and a hydrated form a...
Source: Journal of Molecular Structure - Category: Molecular Biology Source Type: research
In conclusion, the aqueous stem extract of B. alnoides possesses antimalarial properties. A single oral dose of 5000 mg/kg body weight had no significant toxic effects on the function and structure of the kidneys and liver. These results support its use in traditional medicine for the treatment of malaria. PMID: 31814839 [PubMed]
Source: Evidence-based Complementary and Alternative Medicine - Category: Complementary Medicine Tags: Evid Based Complement Alternat Med Source Type: research
Date: Tuesday, 01 07, 2020; Speaker: Daniel Neafsey, Assistant Professor, Harvard T.H. Chan School of Public Health; 5625 Fishers Lane; 5th Floor Conference Room
Source: NIH Calendar of Events - Category: American Health Source Type: events
In this study, the complete gene sequence ofSjFrzb2 was obtained by using 3 ′-rapid amplification of cDNA ends technology.SjFrzb2 transcript levels at different stages ofS. japonicum maturation were evaluated by quantitative real-time RT-PCR analysis.SjFrzb2 was expressed at all developmental stages examined and exhibited the highest transcription level in 7-day-old worms, then gradually decreased during the growth and developmental stages to reach the lowest level at 18  days post-infection.SjFrzb2 gene expression was higher in female worms than in male worms and was significantly higher in female worms from a ...
Source: Veterinary Research - Category: Veterinary Research Source Type: research
Contributors : Lauren M Smith ; Francis Motta ; Garima Chopra ; J K Moch ; Robert R Nerem ; Bree Cummins ; Kimberly E Roche ; Christina M Kelliher ; Adam R Leman ; John Harer ; Tomas Gedeon ; Norman C Waters ; Steven B HaaseSeries Type : Expression profiling by high throughput sequencingOrganism : Plasmodium falciparumThe blood-stage infection of the malaria parasite, Plasmodium falciparum, exhibits a 48-hour developmental cycle that culminates in the synchronous release of parasites from red blood cells, triggering 48-hour fever cycles in the host. This cycle could be driven extrinsically by host circadian processes, or b...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Plasmodium falciparum Source Type: research
Malaria infection can present with a wide variety of symptoms, ranging from mild to severe. Plasmodium falciparum isolates in uncomplicated and severe malaria infections may have different parasite genetic profil...
Source: Malaria Journal - Category: Infectious Diseases Authors: Tags: Research Source Type: research
arimantas Čėnas With the aim to clarify the mechanism(s) of action of nitroaromatic compounds against the malaria parasite Plasmodium falciparum, we examined the single-electron reduction by P. falciparum ferredoxin:NADP+ oxidoreductase (PfFNR) of a series of nitrofurans and nitrobenzenes (n = 23), and their ability to inhibit P. falciparum glutathione reductase (PfGR). The reactivity of nitroaromatics in PfFNR-catalyzed reactions increased with their single-electron reduction midpoint potential (E17). Nitroaromatic compounds acted as non- or uncompetitive inhibitors towards PfGR with respect to NADPH and glutathione...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research
Natural killer (NK) cells are known to be activated during malaria infection, exhibiting both cytokine production and cytotoxic functions. However, NK cells are heterogeneous in their expression of surface activatory and inhibitory receptors which may influence their response to malaria parasites. Here, we studied the surface marker profile and activation dynamics of NK cells during a Controlled Human Malaria Infection in 12 healthy volunteers. Although there was significant inter-patient variability in timing and magnitude of NK cell activation, we found a consistent and strong increase in expression of the activatory rec...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
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