Zebrafish Have an Ethanol-Inducible Hepatic 4-Nitrophenol Hydroxylase that is Not CYP2E1-like

Publication date: Available online 10 July 2017 Source:Environmental Toxicology and Pharmacology Author(s): Jessica H. Hartman, Jordan S. Kozal, Richard T. Di Giulio, Joel N. Meyer Zebrafish are an attractive model organism for toxicology; however, an important consideration in translating between species is xenobiotic metabolism/bioactivation. CYP2E1 metabolizes small hydrophobic molecules, e.g. ethanol, cigarette smoke, and diesel exhaust components. CYP2E1 is thought to only be conserved in mammals, but recent reports identified homologous zebrafish cytochrome P450s. Herein, ex vivo biochemical measurements show that unlike mammals, zebrafish possess a low-affinity 4-nitrophenol hydroxylase (Km ∼0.6 mM) in hepatic microsomes and mitochondria that is inducible only 1.5- to 2-fold by ethanol and is insensitive to 4-methylpyrazole inhibition. In closing, we suggest creating improved models to study CYP2E1 in zebrafish. Graphical abstract
Source: Environmental Toxicology and Pharmacology - Category: Environmental Health Source Type: research