In vivo intrabursal administration of bioactive lipid sphingosine-1-phosphate enhances vascular integrity in a rat model of ovarian hyperstimulation syndrome

AbstractSTUDY QUESTIONCan the bioactive lipid sphingosine-1 phosphate (S1P) act as an endothelial barrier-enhancing molecule and, in turn, restore the vascular integrity and homoeostasis in a rat model of ovarian hyperstimulation syndrome (OHSS).STUDY ANSWERIn vivo administration of S1P may prevent the early onset of OHSS and decrease its severity.WHAT IS KNOWN ALREADYAlthough advances in the prediction and treatment of OHSS have been made, complete prevention has not been possible yet. S1P in follicular fluid from women at risk of developing OHSS are lower in comparison from women who are not at such risk and administration of S1P in an OHSS rat model decreases ovarian capillary permeability.STUDY DESIGN, SIZE, DURATIONWe used an animal model that develops OHSS in immature Sprague-Dawley rats. The rats were randomly divided into three groups: the control group, which was injected with 10 IU of pregnant mare's serum gonadotropin (PMSG), and 10 IU of  hCG 48 h later; the OHSS group, which was injected with excessive doses of PMSG (50 IU/day) for four consecutive days, followed by hCG; and the OHSS + S1P group, which was injected with the same doses of PMSG and hCG as the OHSS group and then treated with 5 μl S1P (1 mM) under the bursa of both ovaries, whereas the other groups of animals received the S1P vehicle.PARTICIPANTS /MATERIALS, SETTING, METHODSRats were killed by decapitation 48 h after the hCG injection for ovary, endometrium and blood collection. The ovaries were w...
Source: Molecular Human Reproduction - Category: Molecular Biology Source Type: research