KNOCKDOWN OF HOST ANTIOXIDANT DEFENSE GENES ENHANCES THE EFFECT OF GLUCANTIME ON INTRACELLULAR Leishmania braziliensis IN HUMAN MACROPHAGES.

KNOCKDOWN OF HOST ANTIOXIDANT DEFENSE GENES ENHANCES THE EFFECT OF GLUCANTIME ON INTRACELLULAR Leishmania braziliensis IN HUMAN MACROPHAGES. Antimicrob Agents Chemother. 2017 May 01;: Authors: Téllez J, Romero I, Soares MJ, Steindel M, Romanha AJ Abstract Leishmaniasis is a neglected tropical disease affecting millions of people worldwide and represents a major public health problem. Information on protein expression patterns and functional roles within the context of Leishmania-infected human monocyte-derived macrophages (MDMs) under drug treatment conditions are essential to understand the role of these cells in leishmaniasis treatment. We analyzed functional changes in expression of human MDM genes and proteins during in vitro infection by Leishmania braziliensis and treatment with Glucantime (Sb(V)) using quantitative polymerase chain reaction (qPCR) arrays, western blotting, confocal microscopy and short-interfering RNA (siRNA) human gene-inhibition assays. Comparison of the results from gene transcription and protein expression analyses revealed that GSTP1, GCLM, GSR, GSS, TXN, and ABCB5 were strongly upregulated at both mRNA and protein levels in human MDMs infected and treated compared to the control group. Subcellular localization studies showed a primarily phagolysosomal location for the ABCB5 transporter, indicating that this protein may be involved in the transport of Sb(V) By inducing a decrease in L. braziliensis intra...
Source: Antimicrobial Agents and Chemotherapy - Category: Microbiology Authors: Tags: Antimicrob Agents Chemother Source Type: research