Naringenin prevents ischemic stroke damage via anti-apoptotic and anti-oxidant effects.

This study aimed to investigate the anti-apoptotic and anti-oxidant effects of naringenin on ischemic stroke. In vitro, cortical neuron cells isolated from the brains of neonatal Sprague-Dawley rats were randomly divided into control, oxygen and glucose deprivation/reperfusion (OGD/Rep), NAR-L, NAR-M and NAR-H groups. MTT and RT-PCR were used for cell proliferation and apoptosis-related proteins analyses. The effects of NAR on the Nrf2 signaling pathway were investigated using transfection approaches. Differences in mitochondrial dysfunction were analyzed by flow cytometry. In vivo, middle cerebral artery occlusion (MCAO) model was prepared and neurological defects and the brain wet/dry (W/D) ratio were assessed and recorded; apoptosis was measured based on the TUNEL assay. Additionally, biochemical indices were detected both in vitro and in vivo. NAR promoted cortical neuron cell proliferation, inhibited apoptosis and oxidative stress, and regulated the localization of Nrf2 protein (P<0.05). Furthermore, silencing and overexpression of Nrf2 affected cortical neuron cell proliferation and apoptosis (P<0.05). In vivo, NAR could alleviate cerebral edema, improve neurological defects, and reduce apoptosis and oxidative stress (P<0.05). These findings demonstrated that NAR could reduce apoptosis and oxidative stress and that Nrf2 signaling pathway is involved in this regulatory process. NAR has health-promoting properties because of its anti-apoptotic and anti-oxidant ef...

Source: Pharmacological Reports - April 28, 2017 Category: Drugs & Pharmacology Authors: Wang K, Chen Z, Huang J, Huang L, Luo N, Liang X, Liang M, Xie W Tags: Clin Exp Pharmacol Physiol Source Type: research