Evaluation of loop-mediated isothermal amplification assay for rapid diagnosis of Acanthamoeba keratitis

Conclusion: PCR and LAMP assays targeting 18S rDNA gene were found particularly suitable for a rapid and accurate diagnosis of AK. LAMP assay takes 2–3 h lesser than PCR, and thus offers a rapid, highly sensitive and specific, simple and affordable diagnostic modality for patients suspected of AK, especially in resource limited settings
Source: Indian Journal of Medical Microbiology - Category: Microbiology Authors: Source Type: research