The Characteristics of Naive-like T Cells in Tumor-infiltrating Lymphocytes From Human Lung Cancer

In this study, we show the characteristics of T-cell subsets in TILs isolated from NSCLC. CD3+ CD8+ CD45RA+ T cells outnumbered CD3+ CD4+ CD45RA+ T cells in CD45RA+ TILs, but it was the opposite in CD45RO+ TILs. Effector memory CD4+ T cells predominated in CD4+ TILs; about 10% of the stem cell-like memory T cells (Tscm) were detected in TILs. To further analyze their functions, we stimulated TILs from NSCLC patients by mitogens to examine cytokine production. Our data demonstrated that naive-phenotype T cells in TILs secret IFN-γ in abundance; TNF-α-producing T cells were significantly increased in TILs; there were more IL-17-expressing CD4+ Tscm cells than other subtypes of CD4+T cells in TILs. Our findings indicate that the CD4+/CD8+ naive-phenotype T cells and Tscm cells in TILs from lung cancer exhibit distinct composition and strong cytokine production. Attributes of Tscm cells from a naive-like T-cell population in TILs are the promising cell type for adoptive cell therapy in human lung cancer.
Source: Journal of Immunotherapy - Category: Allergy & Immunology Tags: Basic Studies Source Type: research