Determination of bacterial etiologic factor on tracheobronchial lavage in relation to clinical signs of bovine respiratory disease.

This study aimed the determination of the occurrence of M. haemolytica, P. multocida and Mycoplasma spp., in relation to clinical signs of respiratory disease. Tracheobronchial lavage samples were collected from 96 (healthy and unhealthy) cattle in the State of São Paulo, Brazil. Mycoplasma spp. (12.5%) and P. multocida (15.50%) were the most prevalent species. Bacillus sp., Staphylococcus sp., Escherichia coli, Klebsiella oxytoca, Pseudomonas aeruginosa and Klebsiella pneumonia were also isolated. Mollicutes (70.83%), M. bovis (2.94%), and M. dispar (38.23%) were identified using conventional PCR. Submassive sound on acoustic percussion of thorax was associated with the absence of Mollicutes (P = 0.025). Whistling (P = 0.076), and coarse crackle (P = 0.046) were associated with the absence of M. dispar. Clear sound on acoustic percussion of thorax was associated with the absence of M. bovis (P = 0.007). Coughing was associated with the presence of P. multocida (P = 0.035/ CI: 1.12-26.89), but its absence was associated with mucopurulent (P = 0.0215/ CI: 1.55-34.5) and mucoid nasal discharge (P = 0.068/ CI: .19-28.5), submassive sound (P = 0.031/ CI: 1.23-75.5), fine crackle (P = 0.058/ CI: 1.23-20.1), and coarse crackle (P = 0.046/ CI: 2.38-70.8). The high prevalence of Pasteurella multocida and Mycoplasma spp. in unhealthy calves increases the importance of these microorganisms in the pathogenesis of the respiratory diseases. This study increases the information about the ...
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research