Third-generation cephalosporin-resistant non-typhoidal Salmonella isolated from human feces in Japan.

Third-generation cephalosporin-resistant non-typhoidal Salmonella isolated from human feces in Japan. Jpn J Infect Dis. 2016 Aug 31; Authors: Saito S, Koori Y, Ohsaki Y, Osaka S, Oana K, Nagano Y, Arakawa Y, Nagano N Abstract Detection and characterization of β-lactamase genes in a total of ten non-typhoidal Salmonella (NTS) clinical isolates exhibiting resistance to the third-generation cephalosporins collected during 2012 -2014 in Japan were conducted. Among five strains with cefotaxime MIC (minimum inhibitory concentration) ≥64 μg/ml and positive results of clavulanic acid inhibition effect, blaCTX-M-2 was detected in three strains (serotypes Stanley and Muenchen), and each of blaTEM-52 (serotype Manhattan) and blaSHV-12 (serotype Infantis) was found in one strain. The blaCMY-2 was detected in all of the remaining five strains (serotypes Infantis, Rissen, Newport, and Saintpaul) with cefotaxime MICs of 4 to 32 μg/ml and positive results of cloxacillin- and 3-aminophenylboronic acid-based inhibition tests. ISEcp1 was located upstream of the blaCMY-2 in four strains and of the blaCTX-M-2 in one strain. Incompatibility (Inc) A/C, Inc P, and Inc I1 plasmids were present in strains harboring blaCMY-2 which were detected predominantly in this study. Acquisition of resistance to the third-generation cephalosporins by invasive NTS may well limit therapeutic options for severe systemic infections, and would cause serious public heal...
Source: Japanese Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: Jpn J Infect Dis Source Type: research