Identification of plum and peach seed proteins by nLC-MS/MS via combinatorial peptide ligand libraries.

Identification of plum and peach seed proteins by nLC-MS/MS via combinatorial peptide ligand libraries.
J Proteomics. 2016 Jul 25;
Authors: González-García E, Marina ML, García MC, Righetti PG, Fasoli E
Abstract
Plum (Prunus domestica L.) and peach (Prunus persica (L.) Batsch) seed proteins are a source of bioactive peptides. These seeds, though, are usual residues produced during canning and beverage preparation that, in most cases, are irreversibly lost. The recovery and identification of these proteins might be of importance in human nutrition. This work employs the combinatorial peptide ligand libraries (CPLLs) technology as a tool to reduce the proteins dynamic concentration range. The most suitable extraction and CPLL capture conditions have been obtained and applied for the comprehensive identification of seed proteins. The analysis of recovered species by nLC-MS/MS has allowed the identification of 141 and 97 unique gene products from plum and peach seeds, respectively. It was possible to identify 16 proteins belonging to the Prunus genus. Moreover, a high number of histones and seed storage proteins were identified. Additionally, 21 and 14 bioactive peptides previously identified were found within protein sequences in plum and peach seeds, respectively.
SIGNIFICANCE: Plums and peaches seeds are cheap sources of proteins that are irretrievably lost after canning and beverage production. Although this kind of residu...

Source: Journal of Proteomics - July 24, 2016 Category: Biochemistry Authors: González-García E, Marina ML, García MC, Righetti PG, Fasoli E Tags: J Proteomics Source Type: research