Gene/protein expression of CAPN1/2-CAST system members is associated with ERK1/2 kinases activity as well as progression and clinical outcome in human laryngeal cancer

This study was aimed to evaluate the CAPN1/2-CAST-ERK1/2 enzyme system mRNA/protein level and to investigate whether they can promote the dynamic of tumor growth and prognosis. The mRNA expression of marker genes was determined in 106 laryngeal cancer (SCLC) cases and 73 non-cancerous adjacent mucosa (NCLM) controls using quantitative real-time PCR. The level of corresponding proteins was analyzed by Western Blot. SLUG expression, as indicator of pathological advancement was determined using IHC staining. Significant increases of CAPN1/2-CAST-ERK1/2 levels of mRNA/protein were noted in SCLC compared to NCLM ( < em class= " a-plus-plus " > p < /em >   & lt;  0.05). As a result, a higher level of < em class= " a-plus-plus " > CAPN1 < /em > and < em class= " a-plus-plus " > ERK1 < /em > genes was related to larger tumor size, more aggressive and deeper growth according to TFG scale and SLUG level ( < em class= " a-plus-plus " > p < /em >   & lt;  0.05). There were also relationships of < em class= " a-plus-plus " > CAPN1/2 < /em > and < em class= " a-plus-plus " > ERK1 < /em > with incidences of local/nodal recurrences ( < em class= " a-plus-plus " > p < /em >   & lt;  0.05). An inverse association for < em class= " a-plus-plus " > CAPN1/2 < /em > , < em class= " a-plus-plus " > CAST < /em > , and < em class= " a-plus-plus " > ERK1/2 < /em > transcripts was determined with regard to overall survival ( < em class= " a-plus-plus " > p < /em >   & lt;  0.05). I...
Source: Tumor Biology - Category: Cancer & Oncology Source Type: research