Class 1 integrons in non-clonal multidrug resistant Acinetobacter baumannii from Iran, description of the new blaIMP-55 allele in In1243.

This study has been conducted to evaluate the detection of metallo-β-lactamase, characterization of class 1 integron and determination of clonal relatedness among A. baumannii hospital isolates. Sixty five clinical isolates of MDR-AB were recovered from two Iranian hospital's intensive care units (ICUs) from February to August 2013. Integrase (intI1) and blaIMP genes were detected in 70.8% (n = 46/65) and 9.23% (n = 6/65) of the isolates using PCR assay, respectively. No other MBL genes (blaVIM, blaSIM, blaNDM) were detected. PCR-sequencing of integron gene cassette revealed the following arrays: blaOXA10-aacA4-blaIMP-55-cmlA5 (as a novel array was designated In1243), aacC1 and aadA1. Analysis of blaIMP gene revealed a new allele designated as blaIMP-55. Gene transfer experiment by conjugation showed the 36kb conjugative plasmid harboring In1243. The clonal assessment by rep-PCR demonstrated a high degree relatedness among the strains but strains harboring In1243 displayed a different rep-PCR profile. In this study we identified a novel class 1 integron (In1243) encoded a new blaIMP allele resided on a transferable plasmid in nonclonal strains of MDR-AB. PMID: 27430533 [PubMed - as supplied by publisher]
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research