Mesenchymal Stromal Cells are Readily Recoverable from Lung Tissue, but not the Alveolar Space, in Healthy Humans

This article is protected by copyright. All rights reserved. Human mesenchymal stromal cells were isolated from healthy parenchymal lung tissue (LT‐MSCs, n=24, 4 samples from 6 patients) and lung allograft bronchoalveolar lavage fluid (BAL‐MSCs, n=12, 1 sample from 12 patients) and gene expression was analysed by microarray. Using Significance Analysis of Microarrays, 105 differentially expressed genes were identified. When visualised as a hierarchical cluster, MSC populations segregate into two distinct groups when samples and probes are clustered using Pearson correlation. Genes that were upregulated in BAL‐MSCs include those associated with cellular migration, transforming growth factor‐β activation and fibroblastic differentiation. Our findings suggested that BAL‐MSCs are a dysregulated population of tissue resident MSCs and may contribute to allograft fibrosis.
Source: Stem Cells - Category: Stem Cells Authors: Tags: Tissue ‐Specific Stem Cells Source Type: research