Memory deficit, toxic effects and activity of Na+, K+-ATPase and NTPDase in brain of Wistar rats submitted to orally treatment with alpha-terpinene

Publication date: Available online 24 June 2016 Source:Environmental Toxicology and Pharmacology Author(s): Matheus D. Baldissera, Carine F. Souza, Thirssa H. Grando, Michele R. Sagrillo, Gerson F. B De Brum, Kátia Nascimento, Diulle S. Peres, Miriãn F. Maciel, Sérgio O. Silveira, Sonia C.A. Da Luz, Pedro H. Doleski, Daniela B.R. Leal, Aleksandro S. da Silva, Silvia G. Monteiro The neurotoxic effects and activity of Na+, K+-ATPase and NTPDase in Wistar rats after treatment with α-terpinene (daily oral administration of 0.5, 0.75 and 1.0mLkg−1 for 10 days) were examined. Results of the inhibitory avoidance task showed a memory deficit (p<0.05) in rats treated with all doses of α-terpinene. The evaluation of DNA damage in brain tissue revealed an increase (p<0.05) on frequency of damage and damage index in all concentrations. According to the cytotoxicity assay, doses of 0.5, 0.75 and 1.0mLkg−1 increase the lactate dehydrogenase levels, and doses of 1.0mLkg−1 also decrease (p<0.05) cell viability in brain cells. A decrease (p<0.05) on Na+, K+-ATPase activity in brain tissue and on NTPDase activity in serum were observed in all concentrations of α-terpinene. These results suggest that the α-terpinene was cytotoxic and genotoxic to the brain cells by inducing loss of cell viability and DNA damage, as well as causing alterations in Na+, K+-ATPase and NTPDase activity, what may contribute to the memory deficit...
Source: Environmental Toxicology and Pharmacology - Category: Environmental Health Source Type: research