Cytokine expression in 3 chicken host systems infected with H9N2 influenza viruses with different pathogenicities.

Cytokine expression in 3 chicken host systems infected with H9N2 influenza viruses with different pathogenicities. Avian Pathol. 2016 May 23;:1-26 Authors: Wang J, Cao Z, Guo X, Zhang Y, Wang D, Xu S, Yin Y Abstract SD/818 and SD/196 are H9N2 influenza virus strains isolated from chickens from the same farm at different times that exhibited similar genetic evolution. However, strain SD/818 exhibited higher pathogenicity in chickens than strain SD/196 and other H9N2 influenza virus epidemic strains from China. The expression of cytokines is an important host defense mechanism following viral infection and their intensity is a major determinant of viral pathogenicity. To elucidate the mechanism underlying the increased pathogenicity of strain SD/818 from the host's perspective, viral replication and cytokine expression were dynamically studied using real-time quantitative reverse transcription PCR in chickens infected with strain SD/818 compared with chickens infected with strain SD/196 in this study. The results showed that the replication of strain SD/818 and the expression of IL-1β, IL-6, TNF-α, IFN-α and IFN-β induced by strain SD/818 were higher than those induced by strain SD/196 in the chicken host system. Expression of these cytokines in chickens were coincided or followed to that of virus replication. These results suggested that high-level viral replication and pro-inflammatory cytokine expression (but not decreased type ...
Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research