Alterations in antioxidant/oxidant gene expression and proteins following treatment of transformed and normal colon cells with tellurium compounds

Publication date: Available online 11 March 2016 Source:Environmental Toxicology and Pharmacology Author(s): Puneet Vij, Diane Hardej The current study evaluated the potential of TeCl4 and DPDT to accumulate within cells and cause oxidative stress. HO-1, antioxidant gene expression and protein alterations were studied. • Significant Te accumulation was observed in HT-29 cells (500–1000μM DPDT; 125–1000μM TeCl4) and CCD-18Co cells (500μM DPDT and TeCl4). • Significant increases in HO-1 were observed with 250–1000μM DPDT and 62.5–1000μM TeCl4 in HT-29 cells and in 500–1000μM DPDT and 62.5–1000μM TeCl4 in CCD-18Co cells. • In CCD-18Co cells, a significant increase in COX-2 was observed at 500–1000μM DPDT and 125–1000μM TeCl4. • Significant increase in NQO1 was observed with exposure to 500–1000μM DPDT and TeCl4. • In HT-29 cells, increased CYGB was noted at concentrations of 500–1000μM DPDT and TeCl4, while significant increases were noted in NCF–1at 1000μM DPDT and TeCl4. • No change in MT-3 and GSR were observed in either cell line.
Source: Environmental Toxicology and Pharmacology - Category: Environmental Health Source Type: research