Nanofiber Scaffolds Support Bone Regeneration Associated with Pulp Stem Cells.

This study reports the bone regeneration potential using biodegradable nanofibers from poly (lactic-co-glycolic acid) (PLGA) in association with human mesenchymal stem cells from dental pulp of deciduous teeth (SCDT). Five samples of SCDT were seeded with scaffolds (test) or without scaffolds (control) for cell adhesion and viability assay. To evaluate the ability of the association in promoting bone formation, critical defects were made in the calvarium of rats (n=20), which were then divided into the following groups: I - sham group; II - implant of scaffolds; III - scaffolds/SCDT; IV - scaffolds/SCDT kept for 13 days in osteogenic media. After 60 days, the histomorphometric analysis was performed. It was observed that the adherence and viability of SCDT in control and test group was similar throughout the experiment (p>0.05). The association of scaffolds/SCDT maintained in osteogenic media, showed greater bone formation than the other groups (p<0.05). The study demonstrated that the association of SCDT seeded in biodegradable PLGA scaffolds has the ability to promote bone regeneration in rats and this is a promising alternative for application in regenerative medicine. PMID: 24588088 [PubMed - as supplied by publisher]
Source: Current Stem Cell Research and Therapy - Category: Stem Cells Authors: Tags: Curr Stem Cell Res Ther Source Type: research