Stroke serum priming modulates characteristics of mesenchymal stromal cells by controlling the expression miRNA-20a.

Stroke serum priming modulates characteristics of mesenchymal stromal cells by controlling the expression miRNA-20a. Cell Transplant. 2016 Jan 13; Authors: Kim EH, Kim DH, Kim HR, Kim SY, Kim HH, Bang OY Abstract Transplantation of mesenchymal stem cells (MSCs), expanded with fetal bovine serum (FBS), has some limitations, including a long culture period required to obtain sufficient stem cells. Priming of MSCs with serum from patients with ischemic stroke (stroke serum) increased the proliferation rate and the neurorestorative capacity of MSCs. We hypothesized that this novel priming method increases the proliferation rate of MSCs via the regulation of microRNAs (miRs). Thus, we investigated miR profiling in stroke serum primed MSCs and tested whether the regulation of certain miRs may affect the proliferation rate of rat MSCs. The proliferation rate of MSCs cultured with stroke serum was higher than that of MSCs cultured with normal serum or FBS. Using miR microarray analysis, we compared the miR expression profiles between MSCs cultured in FBS and in stroke serum. Among miRs associated with cell proliferation, miR-20a was most significantly increased. Similarly, miR-20a was increased in MSCs obtained from the bone marrow of stroke rats compared with MSCs from normal rats. Furthermore, the deregulation of miR-20a by the transfection of MSCs with pre-miR-20a or anti-miR-20a was significantly correlated with the proliferation rate of...
Source: Cell Transplantation - Category: Cytology Authors: Tags: Cell Transplant Source Type: research