The comet assay: Reflections on its development, evolution and applications

Publication date: Available online 29 December 2015 Source:Mutation Research/Reviews in Mutation Research Author(s): Narendra P. Singh The study of DNA damage and its repair is critical to our understanding of human aging and cancer. This review reflects on the development of a simple technique, now known as the comet assay, to study the accumulation of DNA damage and its repair. It describes my journey into aging research and the need for a method that sensitively quantifies DNA damage on a cell-by-cell basis and on a day-by-day basis. That journey led to the comet assay. My inspirations, obstacles and successes on the path to developing this assay and improving its reliability and sensitivity are discussed. Recent modifications and the process of standardizing the technique are also described. What was once untried and unknown has become a technique used around the world for understanding and monitoring DNA damage. The comet assay has already contributed substantially to our understanding of the induction of genetic damage by radiation and chemicals, and its potential extends to many other areas. The comet assay's use has grown exponentially in the new millennium, as emphasis on studying biological phenomena at the single-cell level has increased. I and others have applied the technique across cell types, including germ cells, and in diverse species ranging from mammals and vascular plants to bacteria. As it enters new realms and gains clinical relevance, the comet as...
Source: Mutation Research Reviews in Mutation Research - Category: Genetics & Stem Cells Source Type: research