Does sperm DNA fragmentation affect the developmental potential and the incidence of apoptosis following blastomere biopsy?

Does sperm DNA fragmentation affect the developmental potential and the incidence of apoptosis following blastomere biopsy?
Syst Biol Reprod Med. 2015 Dec 17;:1-10
Authors: Haghpanah T, Salehi M, Ghaffari Novin M, Masteri Farahani R, Fadaei-Fathabadi F, Dehghani-Mohammadabadi M, Azimi H
Abstract
Common methods employed in assisted reproduction technology (ART) include intracytoplasmic sperm injection (ICSI) with an unspecified level of sperm DNA fragmentation (SDF) and preimplantation genetic diagnosis (PGD). The aim of this study was to investigate the impact of SDF on human preimplantation embryo development and the incidence of apoptosis following a single blastomere biopsy. Using sperm chromatin dispersion (SCD) to assess SDF, a total of 20 processed semen samples were categorized into two groups; group I: SDF ≤30% and group II: SDF >30%. After ICSI, fertilization, cleavage, and embryo quality score were assessed. A single blastomere was biopsied from day 3 embryos and development was monitored on day 4. The frequency of apoptosis in biopsied embryos was assayed by TUNEL and the level of BCL-2, BAX, hsa-mir-15a, and hsa-mir-16-1 were assessed by quantitative real-time polymerase chain reaction (qRT-PCR). SCD was found to be negatively correlated with sperm motility and normal form spermatozoa (p < 0.05). The rate of fertilization, cleavage, and embryo quality score were not significantly different between the two grou...

Source: Reproductive Biology - December 17, 2015 Category: Reproduction Medicine Authors: Haghpanah T, Salehi M, Ghaffari Novin M, Masteri Farahani R, Fadaei-Fathabadi F, Dehghani-Mohammadabadi M, Azimi H Tags: Syst Biol Reprod Med Source Type: research