Genotyping of Campylobacter coli strains isolated in Brazil suggests possible contamination among environmental, human, animal and food sources.

Genotyping of Campylobacter coli strains isolated in Brazil suggests possible contamination among environmental, human, animal and food sources. J Med Microbiol. 2015 Nov 2; Authors: Gomes CN, Souza RA, Passaglia J, Duque SS, Medeiros MI, Falcão JP Abstract Campylobacter coli and Campylobacter jejuni are two of the most common causative agents of food-borne gastroenteritis in numerous countries worldwide. In Brazil, campylobacteriosis is under-diagnosed and under-reported, and few studies have molecularly characterized Campylobacter spp. in this country. The current study genotyped 63 C. coli strains isolated from humans (12), animals (21), food (10) and the environment (20) between 1995 and 2011 in Brazil. The strains were genotyped using PFGE, flaA-SVR sequencing and HRMA of the CRISPR locus to better understand C.coli genotypic diversity and compare the suitability of these three methods for genotyping this species. Additionally, the discrimination index (DI) of each of these methods was assessed. Some C. coli strains isolated from clinical and non-clinical origins presented ≥ 80% genotypic similarity by PFGE and flaA-SVR sequencing. HRMA of the CRISPR locus revealed only four different melting profiles. Twenty-two different flaA-SVR alleles were detected. Of these, seven alleles, including gt1647 through gt1653, were classified as novel. The most frequent genotypes were gt30 and gt1647. This distribution reveals the diversity ...
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research