Novel Targeting, lepB Gene, Using Polymerase Chain Reaction with Confronting Two Pair Primers (PCR-CTPP) for Simultaneous Detection of Mycobacterium tuberculosis complex and Mycobacterium bovis.

Novel Targeting, lepB Gene, Using Polymerase Chain Reaction with Confronting Two Pair Primers (PCR-CTPP) for Simultaneous Detection of Mycobacterium tuberculosis complex and Mycobacterium bovis. J Med Microbiol. 2015 Oct 15; Authors: Tharinjaroen CS, Intorasoot S, Anukool U, Phunpae P, Butr-Indr B, Orrapin S, Sangboonruang S, Arunothong S, Chaiyasirinroj B, Kunyanone N, Kasinrerk W, Tragoolpua K Abstract Tuberculosis (TB), caused by members of the Mycobacterium tuberculosis complex (MTC), is the leading cause of infectious disease-related mortality worldwide. The standard method for TB diagnosis usually requires long periods of mycobacteria cultivation, leading to delayed diagnosis, inefficient treatment, and widespread occurrence of the disease. Therefore, a rapid method for the detection and differentiation of MTC from other mycobacteria is essential for disease diagnosis. Here, we describe the potential of using Type I signal peptidase (lepB) gene as a novel target for TB diagnosis based on the confronting two pair primers PCR (PCR-CTPP) that could detect MTC and simultaneously differentiate M. bovis. The limit of detection (LOD) of the developed technique was equivalent to 12-120 bacilli. The PCR-CTPP was highly specific to only MTC and M. bovis and no cross-reaction was detected in the 27 DNA of the nontuberculous mycobacterial and bacterial strains tested. Blinded 39 clinical isolates and 72 sputum samples were used to validate...
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research