The paths toward non-viral CAR-T cell manufacturing: A comprehensive review of state-of-the-art methods

This study aims to investigate the current state-of-the-art in non-viral methods of CAR-T cell manufacturing. In the first part of this study, after reviewing the advantages and disadvantages of the clinical use of viral vectors, different non-viral vectors and the path of their clinical translation are discussed. These vectors include transposons (sleeping beauty, piggyBac, Tol2, and Tc Buster), programmable nucleases (ZFNs, TALENs, and CRISPR/Cas9), mRNA, plasmids, minicircles, and nanoplasmids. Afterward, various methods for efficient delivery of non-viral vectors into the cells are reviewed.PMID:38702027 | DOI:10.1016/j.lfs.2024.122683

Source: Cancer Control - May 3, 2024 Category: Cancer & Oncology Authors: Yekta Metanat Patrik Viktor Ayesha Amajd Irwanjot Kaur Ashraf Mohammed Hamed Noor K Abed Al-Abadi Nathera Hussin Alwan M V N L Chaitanya Natrayan Lakshmaiya Pallavi Ghildiyal Othman Mahjoob Khalaf Carmen Iulia Ciongradi Ioan S ârbu Source Type: research