Development of a gene-coded biosensor to establish a high-throughput screening platform for salidroside production

This study developed a gene-encoded whole-cell biosensor that is specifically responsive to salidroside. The biosensor was created by screening a site-saturated mutagenic library of uric acid response regulatory protein binding bags. This work demonstrates the feasibility of monitoring metabolic flux with whole-cell biosensors for critical metabolites. It provides a promising tool for building salidroside high-yielding strains for high-throughput screening and metabolic regulation to meet industrial needs.PMID:38642492 | DOI:10.1016/j.bbrc.2024.149942
Source: Biochemical and Biophysical Research communications - Category: Biochemistry Authors: Source Type: research