Ultra-Low-Dose UV-C Photo-stimulation Promotes Neural Stem Cells Differentiation via Presenilin 1 Mediated Notch and β-Catenin Activation

In this study, we focused on utilizing ultraviolet-C (UV-C) at a specific wavelength of 254  nm, with an ultra-low dose at intensity of 330 μW/cm2 and a total energy of 594  mJ/cm2 per day over a period of seven days, to stimulate the proliferation and differentiation of mouse neural stem cells (NSCs). The results revealed that the application of ultra-low-dose UV-C yielded the most significant effect in promoting differentiation when compared to mixed ultraviolet (UV) and ultraviolet-A (UV-A) radiation at equivalent exposure levels. The mechanism exploration elucidated the role of Presenilin 1 in mediating the activation of β-catenin and Notch 1 by the UV-C treatment, both of which are key factors facilitating NSCs proliferation and differentiation. These findings introduce a novel approach employing ultra-low-dose UV-C for specifically enhancing NSC differentiation, as well as the underlying mechanism. It would contr ibute valuable insights into brain stimulation and neurogenesis modulation for various diseases, offering potential therapeutic avenues for further exploration.Graphical AbstractUltra-low-dose UV-C photo-stimulation promoted the differentiation of neural stem cells (NSCs) through Presenilin 1 mediated β-catenin and Notch 1 activations.
Source: Molecular Neurobiology - Category: Neurology Source Type: research