Mechanistic studies on protective effects of total flavonoids from Ilex latifolia Thunb. on UVB ‐radiated human keratinocyte cell line (HaCaT cells) based on network pharmacology and molecular docking technique

Network pharmacology and molecular docking were used to predict the active components fromIlex latifolia Thunb. and their possible pathways against UVB radiation. Flavonoids fromIlex latifolia Thunb. were focused as the active components against UVB radiation. PI3K-AKT, JAK-STAT3, NF- κB, and MAPK anti-inflammatory signal pathways were focused as the possible ways for the flavonoids against UVB radiation. Cytological experiment verified the above predictions. AbstractThe aim of the present research is to investigate anti-UVB radiation activity of total flavonoids fromIlex latifolia Thunb. (namely large-leaved Kuding tea) on human keratinocyte cell line (HaCaT cells) based on network pharmacology and molecular docking technique. Network pharmacology was used to screen target genes of active ingredients fromIlex latifolia Thunb. associated with UVB irradiation. The possible signaling pathways were analyzed by KEGG enrichment and verified by cellular experiments. Molecular docking was used to assess the affinity between the active ingredients and the core targets. The prediction of network pharmacology and molecular docking was identified by series experiment in UVB-irradiated HaCaT cells. Network pharmacology results showed that the active ingredients ofIlex latifolia Thunb. for anti-UVB irradiation were mainly flavonoids, and the possible signaling pathways were involved in PI3K-AKT, apoptosis, MAPKs, NF- κB, and JAK-STAT3. Molecular docking indicated key binding activity be...
Source: Photochemistry and Photobiology - Category: Science Authors: Tags: RESEARCH ARTICLE Source Type: research