Generation of two induced pluripotent stem cell lines (LVPEIi007-B, LVPEIi008-B) from patients harboring homozygous mutation in ABCA4 (c.6088C > T) using non-integrative Sendai virus-based approach

Stem Cell Res. 2024 Apr 9;77:103418. doi: 10.1016/j.scr.2024.103418. Online ahead of print.ABSTRACTMutations in ABCA4 gene leads to the most common form of an inherited retinal disease namely, the Stargardt disease, type 1. Here, we report the generation of two different patient-specific induced pluripotent stem cell lines (LVPEIi007-B and LVPEIi008-B), carrying an identical homozygous mutation, (c.6088C>T) within the exon 44 of ABCA4 gene. These lines were generated by the reprogramming of patient-specific dermal fibroblasts, using the integration-free, Sendai viral vectors. Both lines were stably expanded and expressed the stemness and pluripotency markers, differentiated into cell types of all three germ layers, and maintained a normal karyotype.PMID:38615588 | DOI:10.1016/j.scr.2024.103418
Source: Cell Research - Category: Cytology Authors: Source Type: research