An Accurate Assessment of Docosahexaenoic Acid in Laying Hen Serum for Regulatory Studies

The objective of this article is to investigate whether LC-ESI-MS/MS is fit for purpose for the determination of DHA in laying hen serum. The disclosure of this work will be beneficial for researchers investigating poultry enrichment for regulatory and toxicological studies. The method was found to be linear over the range. Precision and accuracy results met acceptance criteria and the Limit of Quantitation (LOQ) was established as 1 µg/mL. Recoveries of DHA were obtained for quality control samples and stability studies were performed. The results of the verification study complimented those of the validation study. In summation, the method was established as fit for purpose for measuring total DHA in laying hen serum.PMID:30636867 | PMC:PMC6318818 | DOI:10.1177/1177390118815146
Source: Analytical Chemistry Insights - Category: Chemistry Authors: Source Type: research