A metagenomics pipeline reveals insertion sequence-driven evolution of the microbiota

Cell Host Microbe. 2024 Mar 21:S1931-3128(24)00085-4. doi: 10.1016/j.chom.2024.03.005. Online ahead of print.ABSTRACTInsertion sequence (IS) elements are mobile genetic elements in bacterial genomes that support adaptation. We developed a database of IS elements coupled to a computational pipeline that identifies IS element insertions in the microbiota. We discovered that diverse IS elements insert into the genomes of intestinal bacteria regardless of human host lifestyle. These insertions target bacterial accessory genes that aid in their adaptation to unique environmental conditions. Using IS expansion in Bacteroides, we show that IS activity leads to the insertion of "hot spots" in accessory genes. We show that IS insertions are stable and can be transferred between humans. Extreme environmental perturbations force IS elements to fall out of the microbiota, and many fail to rebound following homeostasis. Our work shows that IS elements drive bacterial genome diversification within the microbiota and establishes a framework for understanding how strain-level variation within the microbiota impacts human health.PMID:38565143 | DOI:10.1016/j.chom.2024.03.005
Source: Cell Host and Microbe - Category: Microbiology Authors: Source Type: research