A reverse translational approach reveals the protective roles of Mangifera indica in inflammatory bowel disease
In this study, we aimed to dissect the immunomodulatory and anti-inflammatory properties of MIE using a reverse translational approach, by initially using blood from an adult IBD inception cohort and then investigating the mechanism of action in a preclinical model of T cell-driven colitis. Of clinical relevance, MIE modulates TNF-α and IL-17 levels in LPS spiked sera from IBD patients as an ex vivo model of intestinal barrier breakdown. Preclinically, therapeutic administration of MIE significantly reduced colitis severity, pathogenic T-cell intestinal infiltrate and intestinal pro-inflammatory mediators (IL-6, IL-17A, TNF-α, IL-2, IL-22). Moreover, MIE reversed colitis-induced gut permeability and restored tight junction functionality and intestinal metabolites. Mechanistic insights revealed MIE had direct effects on blood vascular endothelial cells, blocking TNF-α/IFN-γ-induced up-regulation of COX-2 and the DP2 receptors. Collectively, we demonstrate the therapeutic potential of MIE to reverse the immunological perturbance during the onset of colitis and dampen the systemic inflammatory response, paving the way for its clinical use as nutraceutical and/or functional food.PMID:38522129 | DOI:10.1016/j.jaut.2024.103181
Source: Journal of Autoimmunity - Category: Allergy & Immunology Authors: Anella Saviano Anna Schettino Nunzia Iaccarino Adel Abo Mansour Jenefa Begum Noemi Marigliano Federica Raucci Francesca Romano Gelsomina Riccardi Emma Mitidieri Roberta d'Emmanuele di Villa Bianca Ivana Bello Elisabetta Panza Martina Smimmo Valentina Vell Source Type: research