Identification of Ultrastructural Details of the Astrocyte Process System in Nervous Tissue of the Brain Using Correlative Scanning Probe and Transmission Electron Microscopy

Dokl Biol Sci. 2024 Mar 2. doi: 10.1134/S0012496623600185. Online ahead of print.ABSTRACTNanoscale morphological features of branched processes of glial cells may be of decisive importance for neuron-astrocyte interactions in health and disease. The paper presents the results of a correlation analysis of images of thin processes of astrocytes in nervous tissue of the mouse brain, which were obtained by scanning probe microscopy (SPM) and transmission electron microscopy (TEM) with high spatial resolution. Samples were prepared and imaged using a unique hardware combination of ultramicrotomy and SPM. Astrocyte details with a thickness of several tens of nanometers were identifiable in the images, making it possible to reconstruct the three-dimensional structure of astrocytic processes by integrating a series of sequential images of ultrathin sections of nervous tissue in the future.PMID:38430297 | DOI:10.1134/S0012496623600185

Source: Doklady Biological Sciences - March 2, 2024 Category: Biology Authors: O I Agapova A E Efimov E A Obraztsova K E Mochalov D O Solovyeva V A Oleinikov I I Agapov S V Gautier Source Type: research