The S1 spike protein of SARS-CoV-2 upregulates the ERK/MAPK signaling pathway in DC-SIGN-expressing THP-1 cells

In this study, we report that the S1 spike protein of SARS-CoV-2 induces the phosphorylation of ERKs in THP-1 cells, a DC-SIGN-expressing human monocytic leukemic cell line. On the other hand, the phosphorylation level of NF-κB remained unchanged under the same conditions. These data suggest that the major cell signaling pathway regulated by S1 spike protein is the ERK pathway, which is superior to the NF-κB pathway in these DC-SIGN-expressing THP-1 cells and may contribute to immune hyperactivation in SARS-CoV-2 infections. Additionally, several glycans such as mannans, mannosylated BSA, the serum amyloid beta protein, and ICAM3 suppressed ERK phosphorylation suggesting that these molecules are target molecules for SARS-CoV-2 infection by suppressing immune hyperactivation that occurs in the ERK signaling pathway.PMID:38453000 | DOI:10.1016/j.cstres.2024.03.002
Source: Cell Stress and Chaperones - Category: Cytology Authors: Source Type: research