In planta transformation in wheat: an improved protocol to develop wheat transformants

CONCLUSIONS: Screening at T0 stage, reduced the load of plants to be taken to T1 generation and their screening thereof at T1 with no overall loss in transformation efficiency. We successfully transformed wheat genotype HD2894 with 3.33% transformation efficiency using a simple, effective method which was less labour-intensive and less time-consuming. This method may be utilized to develop wheat transgenic as well as genome edited lines for desirable traits.PMID:38460010 | DOI:10.1007/s11033-024-09333-8
Source: Molecular Biology Reports - Category: Molecular Biology Authors: Source Type: research